Part:BBa_K4047035

Fructose-1,6-bisphosphatase

The gene encoding fructose-1,6-bisphosphatase class I

Usage and Biology

This coding region was sourced from the Synechococcus elongatus PCC 7942 Genbank genome sequence for fructose-1,6-bisphosphatase (FBPase) to maximize efficiency within the same organism as a chassis. This enzyme is natively expressed within the organism and plays a role in its central metabolism, catalyzing the conversion of fructose-1,6-bisphosphate to fructose-6-phosphate.

This coding region has significant alignment with other categorized Synechococcus strains, as shown in the nucleotide blast result of the provided sequence through https://blast.ncbi.nlm.nih.gov/Blast.cgi (Figure 1).

Figure 1: Nucleotide blast results for fbp

Although this sequence possesses several nucleotide sequences that make it BioBrick incompatible, it is a vital central metabolism gene that future teams may want to work with. Relevant silent mutations could resolve this incompatibility and be a great contribution for future teams.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 493
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 493
  Illegal NotI site found at 746
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 36
  Illegal BglII site found at 656
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 493
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 493
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 22